![]() ![]() 230000003213 activating Effects 0.000 abstract description 7.239000012074 organic phase Substances 0.000 abstract description 9.238000010438 heat treatment Methods 0.000 abstract description 15.BDERNNFJNOPAEC-UHFFFAOYSA-N propanol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 3.239000012224 working solution Substances 0.000 claims description 5.LRHPLDYGYMQRHN-UHFFFAOYSA-N n-butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6.OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6.ZXEKIIBDNHEJCQ-UHFFFAOYSA-N Isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims description 6.239000003960 organic solvent Substances 0.000 claims description 7.IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 7.239000006228 supernatant Substances 0.000 claims description 9.239000000203 mixture Substances 0.000 claims description 9.ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-dimethylformamide Chemical group CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 10.239000007853 buffer solution Substances 0.000 claims description 12.OC()=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 15 UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances.239000011248 coating agent Substances 0.000 claims description 26.238000000576 coating method Methods 0.000 claims description 27.238000007789 sealing Methods 0.000 claims abstract description 5.238000000034 method Methods 0.000 claims abstract description 9.238000005406 washing Methods 0.000 claims abstract description 14.239000000243 solution Substances 0.000 claims abstract description 44.LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 49.238000005859 coupling reaction Methods 0.000 title claims abstract description 36.238000010168 coupling process Methods 0.000 title claims abstract description 36.230000001808 coupling Effects 0.000 title claims abstract description 36.108090001123 antibodies Proteins 0.000 title claims abstract description 53.102000004965 antibodies Human genes 0.000 title claims abstract description 53.239000011324 bead Substances 0.000 title claims abstract description 119.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Zhuhai Livzon Diagnostics Inc filed Critical Zhuhai Livzon Diagnostics Inc Priority to CN201810094598.3A priority Critical patent/CN108279303B/en Publication of CN108279303A publication Critical patent/CN108279303A/en Application granted granted Critical Publication of CN108279303B publication Critical patent/CN108279303B/en Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee Zhuhai Livzon Diagnostics Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor 张晓红 曾敏霞 林燕清 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Active Application number CN201810094598.3A Other languages Chinese ( zh) Google Patents Method for coupling antibody with carboxylated magnetic beadsĭownload PDF Info Publication number CN108279303B CN108279303B CN201810094598.3A CN201810094598A CN108279303B CN 108279303 B CN108279303 B CN 108279303B CN 201810094598 A CN201810094598 A CN 201810094598A CN 108279303 B CN108279303 B CN 108279303B Authority CN China Prior art keywords magnetic beads carboxylated magnetic antibody edc carboxylated Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CN108279303B - Method for coupling antibody with carboxylated magnetic beads CN108279303B - Method for coupling antibody with carboxylated magnetic beads ![]()
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